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Growth kinetics of wild-type M. loti and nodPQ and cysH mutants. (A) Wild-type M. loti (wt) and the nodPQ and cysH mutants were cultured in minimal medium in the absence of exogenous cysteine and methionine. (B) Wild-type M. loti and the nodPQ and cysH mutants were cultured in minimal medium in the presence of 10 μM cysteine and methionine. Aliquots were collected from each culture approximately every 8 h, and their optical densities were measured using spectrophotometry.
