TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference(s) or source |
|---|---|---|
| M. loti strains | ||
| NZP-2235 | Wild-type M. loti | 26 |
| GTO015 | NZP-2235/pMS03 | This study |
| GTO016 | NZP-2235/pMS03::nodPQ | This study |
| GTO100 | NZP-2235 nodPQ::Nmr | This study |
| GTO101 | NZP-2235 nodPQ::Nmr/pMS03::nodPQ | This study |
| GTO108 | NZP-2235 nodPQ::Nmr/pMS03 | This study |
| GTO110 | NZP-2235 cysH::Nmr | This study |
| GTO112 | NZP-2235 nodS::Nmr | This study |
| S. meliloti Rm41 | AK684 Strr | 48 |
| E. coli strains | ||
| MG1655 | Wild-type E. coli | 5 |
| DM63 | cysN96::kan proC leu thi ara gal lac hsd Strr | 36 |
| JM96 | thr-1 leuB(Am) fhuA2 lacY1 glnV44(AS) gal-6 λ−trp-1 hisG1(Fs) rfbC1 cysH56 galP63 Δ(gltB-gltF)500 malT1(λr) xyl-7 mtlA2 ΔargH1 rplL9 thi-1 | 27, 28 |
| CAG12182 | λ−cysI3152::Tn10kan rph-1 | 63 |
| AT2427 | λ− e14− cysJ43 relA1 spoT1 thi-1 creC510 | 65 |
| RL165 | thr-1 leuB(Am) fhuA2 lacY1 glnV44(AS) gal-6 λ−trp-1 hisG1(Fs) cysK511 malT1(λr) xyl-7 mtlA2 ΔargH1 rplL9 thi-1 | 19 |
| GET130 | cysN96::kan proC leu thi ara gal lac hsd Strr/pMS03 | This study |
| GET131 | cysN96::kan proC leu thi ara gal lac hsd Strr/pMS03::nodPQ | This study |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | 58 |
| MT616 | E. coli pRK600 (conjugation helper strain) | 33 |
| Plasmids | ||
| pGTO100 | pJQ200::nodPQ::Nmr (including 500 bp immediately 5′ and 3′ of nodPQ) | This study |
| pGTO101 | pMS03::nodPQ | This study |
| pGTO102 | pDW33::nodP (internal fragment) | This study |
| pGTO110 | pJQ200::cysH::Nmr (including 500 bp immediately 5′ and 3′ of cysH) | This study |
| pGTO112 | pJQ200::nodS::Nmr (including 500 bp immediately 5′ and 3′ of nodS) | This study |
| pGET069 | pJQ200::Nmr | This study |
| pMS03 | Broad-host-range plasmid containing trp promoter | 2 |
| pDW33 | Insertional inactivation plasmid | 14 |
| pCR2.1 | Topo cloning vector | Invitrogen |