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Free DNA is detrimental to the growth of A. baylyi. Competent and noncompetent lineages were grown in minimal medium in the presence of genomic DNA from A. baylyi ΔilvC::Specr-sacB. Dashed lines indicate the range of concentrations of genomic DNA found in the medium after 16 h of growth of ancestral, competent A. baylyi. Curve fits have r2 values of 0.85 and 0.67 for Δcom and wild-type lineages, respectively; shown are means and standard deviations for five lineages.
