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Coomassie blue-stained and UV-illuminated SDS-PAGE gel of membrane fractions of the alkaline phosphatase fusion to the complete nqrB gene (ABNC) and the alkaline phosphatase fusion to the complete nqrC gene (ACNC) in V. cholerae Δnqr. The fluorescent bands indicate the presence of the covalently bound FMNs. Thirty micrograms of membrane protein was used in each lane.
