TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| E. coli LMG194 | F− ΔlacX74 galE thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10 | Invitrogen |
| V. cholerae O395N1 | Wild type, Smr | 25 |
| V. cholerae O395N1 Δnqr | Smr Δnqr | Unpublished results |
| Plasmids | ||
| pBAD-PhoA | pBAD22 derivative containing signal sequenceless phoA with a 5′ KpnI site | 26 |
| pBAD-GFP | pBAD plasmid with cycle 3 gfp | 20 |
| AANC | pBAD-PhoA nqrA; full-length C-terminal fusion | This study |
| ABN240 | pBAD-PhoA nqrB; C-terminal fusion at residue 240 | This study |
| ABN255 | pBAD-PhoA nqrB; C-terminal fusion at residue 255 | This study |
| ABN377 | pBAD-PhoA nqrB; C-terminal fusion at residue 377 | This study |
| ABNC | pBAD-PhoA nqrB; full-length C-terminal fusion | This study |
| ACN90 | pBAD-PhoA nqrC; C-terminal fusion at residue 90 | This study |
| ACN180 | pBAD-PhoA nqrC; C-terminal fusion at residue 180 | This study |
| ACNC | pBAD-PhoA nqrC; full-length C-terminal fusion | This study |
| ADN110 | pBAD-PhoA nqrD; C-terminal fusion at residue 110 | This study |
| ADN125 | pBAD-PhoA nqrD; C-terminal fusion at residue 125 | This study |
| ADNC | pBAD-PhoA nqrD; full-length C-terminal fusion | This study |
| AENC | pBAD-PhoA nqrE; full-length C-terminal fusion | This study |
| AFN80 | pBAD-PhoA nqrF; C-terminal fusion at residue 80 | This study |
| AFN160 | pBAD-PhoA nqrF; C-terminal fusion at residue 160 | This study |
| AFNC | pBAD-PhoA nqrF; full-length C-terminal fusion | This study |
| GANC | pBAD-GFP nqrA; full-length C-terminal fusion | This study |
| GCN90 | pBAD-GFP nqrC; C-terminal fusion at residue 90 | This study |
| GCN180 | pBAD-GFP nqrC; C-terminal fusion at residue 180 | This study |
| GCNC | pBAD-GFP nqrC; full-length C-terminal fusion | This study |
| GDN125 | pBAD-GFP nqrD; C-terminal fusion at residue 125 | This study |
| GDN125+L | PBAD-GFP nqrD; C-terminal fusion at residue 125 with 18-amino-acid linker | This study |
| GDNC | pBAD-GFP nqrD; full-length C-terminal fusion | This study |
| GDNC+L | pBAD-GFP nqrD; full-length C-terminal fusion with 18-amino-acid linker | This study |
| GENC | pBAD-GFP nqrE; full-length C-terminal fusion | This study |