TABLE 2.

Membrane topology predictions among seven Web-based algorithms for each of the six subunits of Na⁺-NQR

Subunit	Predictiona
	TMHMMfix	MEMSAT3	TopPred	TMPred	HMMTOP	DASb	SOUSIb	Consensusc
NqrA	0P	1C	0P	0P	0P	0	0	0
NqrB	9C	10C	10C	8P	11C	9	9	9C
NqrC	1P	1P	2C	3C	2P	1	1	1P
NqrD	5C	6C	5P	5P	6C	6	5	5C
NqrE	6C	5C	6P	6P	6P	6	6	6P
NrqF	1P	1C	3C	2P	2P	2	2	2P

^aValues indicate predicted numbers of transmembrane helices, and the P or C denotes the predicted periplasmic or cytoplasmic C-terminal location, respectively.

^bDAS and SOUSI do not provide predictions of N-terminal locations; therefore, the C-terminal locations cannot be predicted.

^cConsensus model based on rules described previously by Ikeda et al. (18).