FIG. 1.
Diagram of chpA open reading frame detailing the location of phosphotransfer active-site codons (circles), a Tetr insertional deletion spanning codons 812 to 1749 of chpA, and fragments of chpA (clear boxes) that were subcloned to isolate the phosphotransfer codons for mutagenesis. The numbers above the subcloned fragments indicate the codon regions of chpA contained in the fragment. The restriction enzyme sites used for the cassette substitution of the mutated alleles are listed. Full details of the mutagenesis and cloning strategies used to create the mutants referred to in this study may be found in Fig. S1 and Table S1 in the supplemental material, Table 1, and Materials and Methods.