FIG. 5.

Swarming motility phenotypes of chpA point mutants. The photographs are representative of three separate experiments. Swarm plates contained a modified M9 medium with 0.2% glucose (wt/vol) and 0.05% glutamate or aspartate (wt/vol) as sole carbon and nitrogen sources, respectively, and 0.5% agar (Becton Dickinson BBL agar grade A), supplemented with 0.3 mM IPTG and the appropriate antibiotics. Single colonies were inoculated onto the surface of the agar and incubated at 37°C for 16 h (11).