Lysine decarboxylase, cadaverine excretion, and cytosolic SOD activities of wild-type (WT) cells (AR) harboring pRKCadBA in trans after growth transition to LBS (pH 7.5) supplemented with 3 mM MV. (A) The lysine decarboxylase activity of wild-type cells harboring either pRKCadBA or pRK415 was examined 4 h after transfer to LBS (pH 7.5) supplemented with 3 mM MV. Cadaverine in the culture supernatant was determined at the same time points. The error bars correspond to the standard deviations of the means, and cadaverine concentrations represent means ± standard deviations. (B) The cytosolic SOD activity of wild-type cells harboring either pRKCadBA or pRK415 was examined at 2 h, 4 h, and 6 h after the same transfer. The same amount (50 μg) of protein was loaded in each lane.