TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| AR | ATCC 29307, Rifr | 10 |
| JR203 | ATCC 29307, cadA::nptI Kmr | 26 |
| JR309 | AR, ΔcadC | 25 |
| SR1 | AR, soxR::aph Kmr | 13 |
| CSR1 | JR309, ΔcadC soxR::aph Kmr | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 8 |
| S17-1 | C600::RP4 2-(Tc::Mu) (Km::Tn7) thi pro hsdR hsdM+ recA | 30 |
| S17-1λpir | λpir lysogen of S17-1 | 30 |
| BL21(DE3) | E. coli B F−dcm ompT hsdS(rB− mB−) gal λ(DE3) | Stratagene |
| Plasmids | ||
| pDM4 | ori R6K Mob RP4; Cmr | 19 |
| pDMSXR | pDM4 + 3.1-kb fragment containing soxR::aph; Kmr Cmr | 13 |
| pRK415 | ori IncP Mob RP4 lacZα; Tcr | 11 |
| pRKCAT | pRK415 with cat; Smr/Spr Tcr | 13 |
| pCB367 | pRK415 + cadB::cat fusion; 456-bp cadBA DNA from −367 to +89; Smr/Spr Tcr | This study |
| pCB097 | pRK415 + cadB::cat fusion; 186-bp cadBA DNA from −97 to +89; Smr/Spr Tcr | This study |
| pRKCadBA | pRK415 + 3.8-kb cadBA DNA from −10; Tcr | This study |
| pRKCadC | pRK415 + 2.1-kb DNA containing cadC; Tcr | This study |
| pRKSoxR | pRK415 + 0.6-kb DNA containing soxR; Tcr | This study |
| pMAL-p2E | ori M13, MBP fusion vector; Apr | NEB |
| pMAL-SXR | pMAL-p2E + 0.6-kb DNA containing soxR; Apr | This study |