TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5a | F− λ− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) supE44 thi-1 gyrA relA1 | Takara, Kyoto, Japan |
| S17-1 | thi pro hsdR hsdM+recA [chr::RP4-2-Tc::Mu-Km::Tn7] | 26 |
| Chromobacterium violaceum CV026 | Double mini-Tn5 mutant from C. violaceum ATCC 31532; AHL biosenser | 22 |
| Pseudomonas syringae pv. tabaci | ||
| 6605 | Wild type; Nalr | 30 |
| 6605-d3 | 6605 Δorf3 | 33 |
| 6605-dpsyI | 6605 ΔpsyI | This study |
| Plasmids | ||
| pGEM-T Easy | 3.015-kb cloning vector for PCR product; Ampr | Promega, Tokyo, Japan |
| pK18mobsacB | Small mobilizable vector; Kmr; sucrose sensitive (sacB) | 26 |
| pDSK519 | Broad-host-range cloning vector; Kmr | 16 |
| pM3 | 1.69-kb chimeric PCR product deleting orf3 cloned into pK18mobsacB at EcoRI site; Kmr | 33 |
| pDSKGI | 9-kb HindIII fragment containing orf1, orf2, and orf3 genes from P. syringae pv. tabaci 6605 in pDSK519; Kmr | 33 |
a
Ampr, ampicillin resistance; Kmr, = kanamycin resistance; Nalr, nalidixic acid resistance.