FIG. 5.

Potassium permanganate footprints of P_minor (A) and P_{−14A−13A−12T} (B). Reactions were assembled by adding 1.95 μl of a solution containing reconstituted polymerase (Eσ⁷⁰; 0.4 pmol core plus 1.0 pmol of σ⁷⁰) or core alone (0.4 pmol) in protein buffer I to 0.2 pmol of the indicated DNA in 2.05 μl of DNA buffer I. Eσ⁷⁰-dependent bands are marked by arrows and are numbered relative to the P_minor transcriptional +1 site. Nontemplate strand results for P_minor are consistent with the findings of Vuthoori et al. (50), but bands −6 and −4 are referred to as −2 and +1 in that study. Traces for the lanes are shown.