Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pminor requires TGn motif to compensate for a weak −10 element. A denaturing acrylamide gel showing the products of single-round in vitro transcription reactions is overlaid with a graph of the quantitation data. Transcription reactions were assembled and carried out as described in Fig. 2C. The amounts of RNA transcript were determined by densitometry and are shown relative to Pminor, which is set at 100. The values represent the average of three or more transcriptions.
