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Effect of badM on benzoate-CoA ligase activity
| Strainb | Benzoate-CoA ligase activity for cellsa
|
|
|---|---|---|
| Benzoate grown | Succinate grown | |
| CGA009 | 30.2 | 6.0 |
| CGA131 | 27.8 | 55.9 |
| CGA009(pCP561) | 27.7 | 7.5 |
| CGA131(pCP561) | 28.7 | 8.5 |
Expressed as nmol product per min per mg protein in crude cell extracts (8). The concentration of benzoate added to the reaction mixtures was 10 μM. Values are the averages of activities from two independently prepared extracts.
CGA009, wild type; CGA131, badM mutant.
