TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | Host for plasmid amplification | 10 |
| BL21(DE3) | Host for expression of MopAHis and MopBHis | Novagen, Darmstadt, Germany |
| S17-1 | RP4-2 (Tc::Mu) (Km::Tn7) integrated in the chromosome | 27 |
| R. capsulatus | ||
| B10S | Spontaneous Smr mutant of R. capsulatus B10 | 12 |
| KS94A | anfA::[Sp] insertion mutant of B10S | 29 |
| R423AI | mopA::[Gm>] insertion mutant of B10S | 13 |
| R423BI | mopB::[Gm>] insertion mutant of B10S | 13 |
| R423CI | Δ(mopA mopB)::[Gm] deletion mutant of B10S | 13 |
| R438II | mopA::[<Gm] polar insertion mutant of B10S | 29 |
| Plasmids | ||
| pAW12 | pBluescript KS derivative carrying ΔmorABC::[Km] | This study |
| pBluescript KS | High-copy expression vector; Ap | Stratagene, Amsterdam, The Netherlands |
| pBSL86 | Km cassette flanked by polylinker; Ap | 1 |
| pET22b(+) | High-copy His tag expression vector; Ap | Novagen, Darmstadt, Germany |
| pJW32 | pET22b(+) derivative carrying mopAhis | This study |
| pJW33 | pET22b(+) derivative carrying mopBhis | This study |
| pJW42 | pUC18 derivative carrying a mop promoter fragment | This study |
| pJW45 | pUC18 derivative carrying the morA-morC intergenic region | This study |
| pJW59 | pML5 derivative carrying a mop-lacZ transcriptional fusion | This study |
| pKS131A | pPHU236 derivative carrying an anfA-lacZ translational fusion | 13 |
| pML5 | Mobilizable lac fusion broad-host-range vector; Tc | 14 |
| pMOT15 | pML5 derivative carrying a morA-lacZ transcriptional fusion | This study |
| pMOT16 | pML5 derivative carrying a morC-lacZ transcriptional fusion | This study |
| pPHU236 | Mobilizable lac fusion broad-host-range vector; Tc | 11 |
| pSL21I | pML5 derivative carrying a modA-lacZ transcriptional fusion | This study |
| pSL21II | pML5 derivative carrying a mopB-lacZ transcriptional fusion | This study |
| pWKR459 | mob Tc Km | 7 |
a
Ap, ampicillin; Gm, gentamicin; Km, kanamycin; Tc, tetracycline; Sm, streptomycin; Sp, spectinomycin.