TABLE 3.
Expression of Mo-controlled lacZ reporter fusions in R. capsulatus wild-type and mutant strains
| Strain(plasmid) | Genetic background | Reporter fusion | β-Galactosidase activitya
|
|||
|---|---|---|---|---|---|---|
| +Mo/−N | −Mo/−N | +Mo/+N | −Mo/+N | |||
| B10S(pKS131A) | Wild type | anfA-lacZ | 1 ± 1 | 63 ± 10 | 1 ± 1 | 0 ± 0 |
| R423AI(pKS131A) | mopA | anfA-lacZ | 1 ± 1 | 73 ± 11 | 1 ± 1 | 0 ± 0 |
| R423BI(pKS131A) | mopB | anfA-lacZ | 0 ± 0 | 64 ± 10 | 0 ± 0 | 0 ± 0 |
| R423CI(pKS131A) | Δ(mopAB) | anfA-lacZ | 72 ± 18 | 67 ± 9 | 2 ± 2 | 1 ± 1 |
| B10S(pSL21I) | Wild type | modA-lacZ | 11 ± 1 | 70 ± 7 | 6 ± 2 | 4 ± 3 |
| R423AI(pSL21I) | mopA | modA-lacZ | 7 ± 9 | 88 ± 5 | 7 ± 2 | 3 ± 2 |
| R423BI(pSL21I) | mopB | modA-lacZ | 6 ± 5 | 78 ± 11 | 13 ± 6 | 6 ± 0 |
| R423CI(pSL21I) | Δ(mopAB) | modA-lacZ | 87 ± 9 | 89 ± 13 | 0 ± 0 | 2 ± 2 |
| B10S(pSL21II) | Wild type | mopB-lacZ | 21 ± 3 | 22 ± 2 | 18 ± 2 | 18 ± 1 |
| R423AI(pSL21II) | mopA | mopB-lacZ | 18 ± 3 | 18 ± 4 | 18 ± 4 | 23 ± 7 |
| R423BI(pSL21II) | mopB | mopB-lacZ | 16 ± 1 | 22 ± 1 | 16 ± 2 | 18 ± 4 |
| R423CI(pSL21II) | Δ(mopAB) | mopB-lacZ | 17 ± 4 | 20 ± 4 | 21 ± 2 | 20 ± 2 |
| B10S(pMOT15) | Wild type | morA-lacZ | 43 ± 3 | 369 ± 28 | 37 ± 3 | 117 ± 3 |
| R423AI(pMOT15) | mopA | morA-lacZ | 51 ± 4 | 441 ± 52 | 48 ± 3 | 67 ± 27 |
| R423BI(pMOT15) | mopB | morA-lacZ | 120 ± 19 | 408 ± 44 | 70 ± 11 | 171 ± 7 |
| R423CI(pMOT15) | Δ(mopAB) | morA-lacZ | 452 ± 66 | 435 ± 63 | 175 ± 19 | 202 ± 21 |
| B10S(pMOT16) | Wild type | morC-lacZ | 438 ± 27 | 733 ± 76 | 148 ± 14 | 387 ± 39 |
| R423AI(pMOT16) | mopA | morC-lacZ | 411 ± 48 | 791 ± 84 | 147 ± 13 | 368 ± 10 |
| R423BI(pMOT16) | mopB | morC-lacZ | 454 ± 32 | 785 ± 61 | 196 ± 18 | 456 ± 34 |
| R423CI(pMOT16) | Δ(mopAB) | morC-lacZ | 891 ± 63 | 862 ± 56 | 388 ± 36 | 494 ± 41 |
| B10S(pJW59) | Wild type | mop-lacZ | 551 ± 51 | 40 ± 15 | 316 ± 40 | 64 ± 5 |
| R423AI(pJW59) | mopA | mop-lacZ | 24 ± 3 | 25 ± 20 | 41 ± 5 | 43 ± 3 |
| R423BI(pJW59) | mopB | mop-lacZ | 382 ± 18 | 18 ± 4 | 333 ± 22 | 22 ± 2 |
| R423CI(pJW59) | Δ(mopAB) | mop-lacZ | 44 ± 13 | 36 ± 11 | 57 ± 17 | 43 ± 5 |
a
R. capsulatus strains were grown under phototrophic conditions in AK-NL minimal medium either without addition of Mo (−Mo) or in the presence of 10 μM Na2MoO4 (+Mo). N-sufficient or N-limiting conditions were achieved by addition of either 15 mM (NH4)2SO4 (+N) or 9.5 mM serine (−N), respectively. β-Galactosidase activity is given in Miller units (19). Results represent the means and standard deviations of three independent measurements.