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. Author manuscript; available in PMC: 2007 Mar 20.
Published in final edited form as: J Comp Neurol. 2006 Mar 20;495(3):299–313. doi: 10.1002/cne.20881

Fig. 3.

Fig. 3

D2 and CB1 localization in dendritic spines. A–C: Acb core, processed for D2-peroxidase and CB1-gold immunocytochemistry. In A, CB1 gold-silver labeling (small arrows) is located in the subsynaptic region of a dendritic spine (CB1-s). In B, the peroxidase D2 labeling is diffusely distributed, but particularly evident (block arrow) along perisynaptic portions of the plasma membrane distant from the immunogold-silver CB1 labeling (small arrow) in the same dendritic spine. In C, the peroxidase (block arrow) and gold (small arrow) labeling are located in proximity to each other on the perisynaptic membrane. The spines in A–C receive asymmetric synapses (curved arrows) from unlabeled terminals (Ut). D,E: Acb shell, processed for D2-peroxidase and CB1-gold. Peroxidase (block arrows) and gold-silver (small arrows) show partially overlapping aggregated cytoplasmic distributions near endomembranes (em) in dually labeled den-drites (D2/CB1-d). In D, the aggregates are seen near the base of a peroxidase-labeled dendritic spine (D2-s). Scale bars = 0.5 μm.