TABLE 2.
Epidemiological data, resistance determinants, and plasmid backbones of the different clones of E. coliand K. pneumoniae producing CTX-M-15 or CTX-M-16 β-lactamase at the Charles Nicolle Hospital in Tunis
| Clone (n) | CTX-M allele | Period of isolation (mo/yr) | Ward(s) | No. of clones with indicated type of infection:
|
No. of clones witha:
|
Plasmid backbone(s)b | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Urine | Blood | Pus | Respiratory | Catheter | blaTEM-1 | blaOXA-1 | aac(3)-II | aac(6′)-Ib | tetA | sul1 | sul2 | Cipr | |||||
| E1 (10) | 15 | 03/00-01/02 | Surgery, medicine 1, urology | 9 | 0 | 1 | 0 | 0 | 10 | 8 | 9 | 10 | 10 | 0 | 10 | 10 | P1 (2/2) |
| E2 (3) | 16 | 07/01-12/01 | Pediatric | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | P3 (3/3) |
| E3 (3) | 15 | 09/01 | Medicine 1 | 3 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 3 | 0 | 0 | 2 | 3 | P1 (3/3) |
| E4 (1) | 16 | 03/02 | Pediatric | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | P3 (1/1) |
| E5 (1) | 15 | 10/02 | Surgery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | P1 (1/1) |
| E6 (16) | 15 | 12/02-06/03 | Surgery, medicine 1 and 2, urology | 4 | 5 | 3 | 2 | 2 | 15 | 15 | 15 | 15 | 0 | 14 | 0 | 16 | P1 (2/2), P5 (1/1) |
| E7 (1) | 15 | 12/02 | Medicine 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | P1 (1/1) |
| K1 (2) | 16 | 05/00-06/00 | Pediatric | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 2 | 0 | 0 | P3 (2/2) |
| K2 (1) | 16 | 08/01 | Pediatric | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | P3 (1/1) |
| K3 (1) | 15 | 05/02 | Medicine 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | P6 (1/1) |
| K4 (17) | 15 | 10/02-06/03 | Surgery, medicine 1, urology, ICU | 4 | 8 | 4 | 2 | 1 | 14 | 17 | 17 | 17 | 0 | 1 | 17 | 17 | P1 (5/5) |
| K5 (1) | 15 | 12/02 | Urology | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | P1 (1/1) |
| K6 (2) | 15 | 12/02-03/03 | Surgery, medicine 1 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 2 | 0 | 2 | P1 (2/2) |
| K7 (1) | 15 | 03/03 | Pedriatric | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | P7 (1/1) |
| K8 (1) | 15 | 03/03 | Surgery | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | P2 (1/1) |
| K9 (1) | 15 | 05/03 | ICU | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | P4 (1/1) |
a
These resistances were detected by PCR as described in Materials and Methods, except for the resistance to ciprofloxacin (Cipr), which was detected only by the disk diffusion method.
b
The plasmid backbone has been analyzed only in transconjugants or electroporants. Values in parentheses are numbers of plasmid analyses/numbers of transconjugants or electroporants.