TABLE 7.
Comparison of different methods for detecting dhps SNPs in mixed population of P. falciparum parasites with different genotypes (DNA stock equal to 400 parasites/μl for each strain)a
| Codon | Parasite proportion (%)
|
Pyro
|
RFLP
|
||||
|---|---|---|---|---|---|---|---|
| HB3 | K1 | W2 | Amino acid code | No. of expts with positive reactions | Amino acid code | No. of expts with positive reactions | |
| 437 | 100 | 0 | A | 3 | A | 3 | |
| 95 | 5 | A | 2 | A | 3 | ||
| A/G | 1 | ||||||
| 90 | 10 | A/G | 3 | A | 3 | ||
| 85 | 15 | A/G | 3 | A/G | 3 | ||
| 80 | 20 | A/G | 3 | A/G | 3 | ||
| 70 | 30 | A/G | 3 | A/G | 3 | ||
| 60 | 40 | A/G | 3 | A/G | 3 | ||
| 50 | 50 | A/G | 3 | A/G | 3 | ||
| 40 | 60 | A/G | 3 | A/G | 3 | ||
| 30 | 70 | A/G | 3 | A/G | 3 | ||
| 20 | 80 | A/G | 3 | A/G | 3 | ||
| 15 | 85 | A/G | 3 | A/G | 3 | ||
| 10 | 90 | A/G | 3 | A/G | 3 | ||
| 5 | 95 | G | 3 | A/G | 3 | ||
| 581 | 100 | 0 | A | 3 | A | 3 | |
| 95 | 5 | A | 3 | A/G | 3 | ||
| 90 | 10 | A/G | 3 | A/G | 3 | ||
| 85 | 15 | A/G | 3 | A/G | 3 | ||
| 80 | 20 | A/G | 3 | A/G | 3 | ||
| 70 | 30 | A/G | 3 | A/G | 3 | ||
| 60 | 40 | A/G | 3 | A/G | 3 | ||
| 50 | 50 | A/G | 3 | A/G | 3 | ||
| 40 | 60 | A/G | 3 | A/G | 3 | ||
| 30 | 70 | A/G | 3 | A/G | 3 | ||
| 20 | 80 | A/G | 3 | A/G | 3 | ||
| 15 | 85 | A/G | 3 | A/G | 2 | ||
| G | 1 | ||||||
| 10 | 90 | A/G | 3 | G | 3 | ||
| 5 | 95 | G | 3 | G | 3 | ||
| 0 | 100 | G | 3 | G | 3 | ||
| 436 | 100 | 0 | S | 3 | S | 3 | |
| 95 | 5 | S/F | 3 | S | 3 | ||
| 90 | 10 | S/F | 3 | S | 3 | ||
| 85 | 15 | S/F | 3 | S/F | 3 | ||
| 80 | 20 | S/F | 3 | S/F | 3 | ||
| 70 | 30 | S/F | 3 | S/F | 3 | ||
| 60 | 40 | S/F | 3 | S/F | 3 | ||
| 50 | 50 | S/F | 3 | S/F | 3 | ||
| 40 | 60 | S/F | 3 | S/F | 3 | ||
| 30 | 70 | S/F | 3 | S/F | 3 | ||
| 20 | 80 | S/F | 2 | S/F | 3 | ||
| F | 1 | ||||||
| 15 | 85 | F | 3 | S/F | 3 | ||
| 10 | 90 | F | 3 | F | 3 | ||
| 5 | 95 | F | 3 | F | 3 | ||
| 0 | 100 | F | 3 | F | 3 | ||
| 613 | 100 | 0 | A | 3 | A | 3 | |
| 95 | 5 | A/S | 3 | A | 3 | ||
| 90 | 10 | A/S | 3 | A | 3 | ||
| 85 | 15 | A/S | 3 | A/S | 3 | ||
| 80 | 20 | A/S | 3 | A/S | 3 | ||
| 70 | 30 | A/S | 3 | A/S | 3 | ||
| 60 | 40 | A/S | 3 | A/S | 3 | ||
| 50 | 50 | A/S | 3 | A/S | 3 | ||
| 40 | 60 | A/S | 3 | A/S | 3 | ||
| 30 | 70 | A/S | 3 | A/S | 3 | ||
| 20 | 80 | A/S | 3 | A/S | 3 | ||
| 15 | 85 | A/S | 3 | A/S | 3 | ||
| 10 | 90 | S | 3 | A/S | 3 | ||
| 5 | 95 | S | 3 | A/S | 3 | ||
| 0 | 100 | S | 3 | S | 3 | ||
a
Each concentration was tested in triplicate. See Table 4 for abbreviations and definitions.