TABLE 1.
VNTR loci S. enterica serovar Typhimurium strain LT2 and primers selected for PCR
| VNTR primera | 5′ End primer | Sequence (5′ to 3′) | PCR product size (bp) | RU size (bp) | Locus size (bp [no. of RUs]) |
|---|---|---|---|---|---|
| 0819457L | 819392 | ATCCAAGGGGTCGTTAGCTC | 716 | 155 | 558 (3.6) |
| 0819457R | 820108 | ACGTAGCCCCGTATCTGATG | |||
| 2341937L | 2341885 | GTGGGTTCCCTTCCAGATTT | 712 | 60 | 618 (10.3) |
| 2341937R | 2342597 | TTACCCGCGCATATTACCAC | |||
| 2531837L | 2531810 | CCCTATAATGCGACTCCACA | 400 | 117 | 316 (2.7) |
| 2531837R | 2532210 | GAACCTGCGACCAATTGATTA | |||
| 2628542L | 2628422 | GCCATCGGCATTACGATACT | 363 | 36 | 144 (4) |
| 2628542R | 2628785 | GAGGTTAACGCCGGTCTGTA | |||
| 2730867L | 2730843 | TGCTGAGCATAAACGACATCA | 168 | 6 | 82 (13.7) |
| 2730867R | 2731011 | CAGCACCAGAAGCAATGGTA | |||
| 3184543L | 3184463 | TTATTATTCTGAGCACCGCGT | 205 | 6 | 78 (13) |
| 3184543R | 3184668 | TGATACGCTTTTGACGTTGC | |||
| 3414090L | 3414027 | AATTAATTGCCGGATGGTGA | 841 | 116 | 754 (6.5) |
| 3414090R | 3414868 | AGCGATTGCTGGCCTAGAT | |||
| 3629542L | 3629305 | GAAAAACGCGCAAAACTCTC | 751 | 33 | 360 (10.9) |
| 3629542R | 3621111 | GCCACTGGTTGTCCTGTTCT | |||
| 4596419L | 4596165 | TGTTGTGGCACCGTGCTAT | 892 | 232 | 533 (2.3) |
| 4596419R | 4597058 | CGCCCTGGGATAAGTCTGT | |||
| 4810628L | 4810568 | TTGGCAATGATGAACTGGAA | 521 | 118 | 330 (2.8) |
| 4810628R | 4811089 | GCAAAGCGTCTTATCCAACC |
a
Primers are named according to the nucleotide at the 5′ end of the VNTR locus