FIG. 3.

Comparative sensitivity of RT-LAMP and RT-PCR for the detection of JEV. (A). Sensitivity of the JEV RT-LAMP assay as monitored by agarose gel analysis. Lane M, 100-bp DNA ladder (Sigma); lanes 1 to 6, different concentrations of virus, ranging from 10⁴ to 0.1 PFU in a serial 10-fold dilution pattern; lane 7, negative control having no template. The detection limit for the RT-LAMP assay was 0.1 PFU. (B) Sensitivity of RT-PCR as observed by a agarose gel analysis revealing a 162-bp amplicon specific for the NS3 gene of JEV with a detection limit of 10 PFU.