TABLE 1.
Details of RT-LAMP E gene primer sets designed for rapid and real-time detection of JEV
| Primer name and primer component | Genome positiona | Length of oligonucleotide (bp) | Sequence |
|---|---|---|---|
| F3 | 990-1008 | 19 | GGAATGGGCAATCGTGACT |
| B3 | 1224-1206 | 19 | CGTTGTGAGCTTCTCCAGT |
| FIP (F1c+TTTT+F2) | |||
| F1c | 1109-1088 | 22 | GCGGACGTCCAATGTTGGTTTG |
| F2 | 1029-1046 | 18 | GCCACTTGGGTGGACTTG |
| BIP (B1+TTTT+B2c) | |||
| B1 | 1123-1144 | 22 | AAGCTAGCCAACTTGCTGAGGT |
| B2c | 1187-1168 | 20 | CGTCGAGATGTCAGTGACTG |
| FLP | 1087-1070 | 18 | TCGTTTGCCATGATTGTC |
| BLP | 1147-1166 | 20 | GAAGTTACTGCTATCATGCT |
a
Prototype strain JaOArS982 (accession no. M18370).