Colony formation of H. pylori 26695 on different agar media. HB, horse blood agar plate; NISSUI, NISSUI plate Helicobacter; BS, BHI agar with untreated BS; H-BS, BHI agar with heat-treated BS; FBS, BHI agar with untreated FBS; H-FBS, BHI agar with heat-treated FBS. The number of colonies on each agar plate was counted, and the results are expressed as means plus the standard deviation from three plates.