Skip to main content
. 2006 Oct 11;44(12):4541–4544. doi: 10.1128/JCM.01141-06

FIG. 1.

FIG. 1.

LightCycler assay for UL97 codon 460. (A) Detection of mutations in codon 460 for laboratory strains (AD169 and Towne) and a mutant strain (pp6). (B) Semiquantitative detection of mixed viral populations in codon 460. (C) For comparison, RFLP results of the different mutations in codon 460 (6) are given. Panel I shows an NlaIII digest to discriminate between wild-type and mutant strains (lane M, marker; lane 1, AD169; lane 2, pp6; lane 3, GCV-resistant isolate [288128]) from a hematopoietic stem cell transplant recipient with mixed viral populations. Panel II shows an MseI digest to discriminate between different mutations in codon 460 (lane M, marker; lane 1, AD169; lane 2, pp6; lane 3, M460I mutant [plaque purified from strain pp6; pp6*M460I] and lane 4, M460V mutant [plaque purified from strain pp6; pp6*M460V]). (D) Corresponding sequences of wild-type and mixed mutant strains (288128) in codon 460 are shown. Tm, melting temperature; NTC, no template control.