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Light Cycler assay for codon 520. (A) Detection of the H520Q mutation using melting point analysis. (B) AluI digest for the detection of the H520Q mutation by RFLP analysis (12). Lane M, marker; lane 1, AD169; lane 2, p520mut plasmid. (C) Corresponding sequence analysis is shown. Tm, melting temperature; NTC, no template control.
