TABLE 1.
Sequences of HCMV UL97 primers and hybridization probes for the LightCycler assay for codons 460/520 and the site-directed mutagenesis of mutation H520Q
| Primer or probe designation (nucleotide position) | Function | Sequence (5′-3′)a |
|---|---|---|
| 405F (141693-141712) | Forward primer | CTGCTGCACAACGTCAAGGT |
| 550R (142131-142153) | Reverse primer | CCCAGCGCCGACAGCTCCGACAT |
| 460Sensor (141853-141874) | Hybridization probe | ATGAGCACGTTCATGGGTGTAA-Fl |
| 460Anchor (141823-141851) | Hybridization probe | LC640-GTCAAAGTGGCATACACGACACTGGTGAT-P |
| 520Sensor (142032-142053) | Hybridization probe | GAATGTTACCACCCTGCTTTCC-Fl |
| 520Anchor (142055-142076) | Hybridization probe | LC705-ACCCATGCCGCTGCAGAAGCTG-P |
| m520F (142031-142049) | Forward mismatch primer | CGAATGTTACCAGCCTGCTT |
| m520R (142031-142049) | Reverse mismatch primer | AAGCAGGCTGGTAACATTCG |
a
Fl, fluorescein; LC640, fluorescence dye LC Red dye 640; LC705, fluorescence dye LC Red dye 705. Underlined, boldface residues indicate sequence mismatches.