FIG. 1.
Quality control of CFP10. Fifty micrograms of recombinant protein was loaded onto reverse-phase C4 columns and eluted by using a linear acetonitrile gradient. The elution profile was monitored by continuously measuring the absorbance at 214 nm (mAU, milli-absorbance units) (A). Over the 0 to 100% elution gradient, 20 fractions were collected and applied onto SDS-polyacrylamide gels, followed by silver staining. Lanes M, SDS-polyacrylamide gel electrophoresis size marker (97, 66, 45, 31, 21, and 14 kDa); lanes 1 to 20, the 20 eluted fractions, respectively. Fraction 1 corresponds to 0% acetonitrile on the chromatogram, and fraction 20 corresponds to 100% acetonitrile (B).