TABLE 1.
Characteristics of proteins used in this study
| Protein | M. bovis Mb no.a | M. tuberculosis Rv no.b | Present in BCG | % Homology in M. avium subsp. aviumc | Protein size (no. of amino acids) | Comment |
|---|---|---|---|---|---|---|
| ESAT6 | Mb3905 | Rv3875 | No | 29 | 94 | 6-kDa early secretory antigenic target |
| CFP10 | Mb3904 | Rv3874 | No | 24 | 100 | Exported protein cotranscribed with Rv3875 |
| PE13 | Mb1227 | Rv1195 | Yes | 54 | 99 | PE family protein |
| MPB70 | Mb2900 | Rv2875 | Yes | 25 | 193 | Major secreted immunogenic protein MPT70 |
| TB10.4 | Mb0296 | Rv0288 | Yes | 79 | 96 | Low-molecular-wt protein antigen 7 |
| PE5 | Mb0293 | Rv0285 | Yes | 88 | 102 | PE family protein |
| TB27.4 | Mb3908 | Rv3878 | No | 24 | 280 | Conserved hypothetical alanine-rich protein |
a
The numbering system for Mycobacterium bovis strain AF2122/97 was used (16).
b
The Mycobacterium tuberculosis numbering system for laboratory strain H37Rv was used (11).
c
TBLASTN with a blosum 62 scoring matrix. To allow homology searches in regions of low complexity, searches were done without a filter.