FIG. 2.

Detection of chimerical VARV DNA by TaqMan real-time PCR. Amplification plots (cycle number versus delta Rn) of serial 10-fold dilutions (10⁶ to 10 copies per tube) of chimerical VARV DNA tested in triplicate in the same experiment. NC is the negative control. Rn, the normalized reporter signal, is the fluorescence signal of the reporter dye divided by the fluorescence signal of the passive, internal reference dye. Delta Rn is determined by the formula Rn⁺ − Rn⁻, where Rn⁺ is the Rn value for a reaction involving all components, including the template, and Rn⁻ is the value for an unreacted sample.