TABLE 1.
Primer and probe sequences
| Primer or probe | Sequencea |
|---|---|
| Formia 1 | 5′-28593CGTGTAACACGACTCACAATAGAATCT28619 |
| Formia 3 | 5′-28788YGGAAGAGACGGTGTRAGAATATGT28766 |
| VAR130 | 5′-FAM-28722ACACGTCTGTTGGAGACG28739-MGB-NFQ |
| OPOX143 | 5′-VIC-28735AGACGTCATCTGTTCTC28751-MGB-NFQ |
| IC | 5′-NED-CCAGCACACATGTGTCTACT-MGB-NFQ |
| ICSb | 5′-CGTGTAACACGACTCACAATAGAATCTCCAGCACACATGTGTCTACTAATAAAAGTTACAGAATAT |
| ICAb | 5′-CTAATAAAAGTTACAGAATATTTTTCCATAAGTTTTTTAACATATTCTTACACCGTCTCTTCCG |
a
The sequence positions (subscript numbers) correspond to VARV Somalia-1977 (GenBank accession number U18341).
b
ICS and ICA, sense and antisense primers, respectively, for constructing an internal control.