. 2005 Oct 25;44(12):4464–4470. doi: 10.1128/JCM.00276-06

TABLE 2.

Second orthopoxvirus PCR European quality control panel

Sample no.^a or source	Contents^b	Ct value^c for:			Results of this study for^d:
Sample no.^a or source	Contents^b	OPOX143	VAR130	IC	OPOX	VARV
ENIVD02#07	MPXV	24.98	Undet	Undet	Pos	Neg
ENIVD02#10	MPXV	29.10	Undet	Undet	Pos	Neg
ENIVD02#08	MPXV	32.21	Undet	42.94	Pos	Neg
ENIVD02#05	MPXV	36.33	Undet	41.64	Pos	Neg
ENIVD02#20	MPXV	36.70	Undet	42.14	Pos	Neg
ENIVD02#01	MPXV	Undet	Undet	41.32	Neg	Neg
ENIVD02#14	Inh f + MPXV	Undet	Undet	Undet	Inh	Inh
ENIVD02#15	Inh f + MPXV	Undet	Undet	Undet	Inh	Inh
ENIVD02#17	CPXV	41.76	Undet	Undet	Pos	Neg
ENIVD02#13	VACV	31.93	Undet	Undet	Pos	Neg
ENIVD02#12	Tanapox virus	Undet	Undet	40.53	Neg	Neg
ENIVD02#19	Parapox virus	Undet	Undet	40.60	Neg	Neg
ENIVD02#06	Inh f	Undet	Undet	Undet	Inh	Inh
ENIVD02#09	Inh f	Undet	Undet	Undet	Inh	Inh
ENIVD02#02	Neg	Undet	Undet	40.69	Neg	Neg
ENIVD02#03	Neg	Undet	Undet	40.84	Neg	Neg
ENIVD02#04	Neg	Undet	Undet	41.64	Neg	Neg
ENIVD02#11	Neg	Undet	Undet	41.35	Neg	Neg
ENIVD02#16	Neg	Undet	Undet	40.41	Neg	Neg
ENIVD02#18	Neg	Undet	Undet	40.28	Neg	Neg
This study	VARV (1 × 10⁶)	27.79	24.78	Undet	Pos	Pos
This study	VARV(1 × 10⁵)	31.72	28.54	Undet	Pos	Pos
This study	VARV (1 × 10⁴)	35.59	32.21	Undet	Pos	Pos
This study	VARV (1 × 10³)	39.89	36.08	44.66	Pos	Pos
This study	VARV (1 × 10²)	41.37	40.97	41.82	Pos	Pos
This study	VARV (1 × 10¹)	Neg	Neg	41.01	Neg	Neg
This study	VARV (1)	Neg	Neg	41.55	Neg	Neg

Sample number from the second orthopoxvirus PCR European quality control panel performed by ENIVD.

Inh f, inhibitor factors; Neg, no virus. Concentrations (copies per tube) of chimerical VARV DNA obtained in this study are in parentheses.

Ct is the average cycle at which the fluorescence crossed the threshold; values are geometric means of triplicate experiments. Undet, undetectable.

Results obtained by using this real-time PCR assay. OPOX, orthopoxvirus; pos, positive result; neg, negative result; Inh, inhibition.