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. 2006 Oct 4;44(12):4553–4558. doi: 10.1128/JCM.01887-06

TABLE 2.

Melting peak (Tm) and direct DNA sequencing results for four QRDR positions in 22 LVX-resistant S. pneumoniae strains

Straina LVX MIC (μg/ml) ParC codon 79
ParC codon 83
GyrA codon 81
GyrA codon 85
Tm (°C) Sequenceb Tm (°C) Sequenceb Tm (°C) Sequenceb Tm (°C) Sequenceb
ATCC 49619 0.5 60.4 TCT/Ser 56.7 GAT/Asp 52.3 TCC/Ser 56.8 GAA/Glu
N001 8 50.2 TTT/Phe 56.6 WTf 52.3 WT 56.8 WT
N002 8 61.0 WT 56.8 WT 45.4 TTC/Phe 56.0 WT
N003 8 50.1 TTT/Phe 56.7 WT 52.3 WT 50.2 AAA/Lys
N004 16 50.8 TTT/Phe 56.4 WT 51.4 WT 49.7 AAA/Lys
N005 16 50.1 TTT/Phe 56.8 WT 52.1 WT 50.2 AAA/Lys
N006 8 50.1 TTT/Phe 56.8 WT 52.1 WT 56.4 WT
N007 16 50.4 TTT/Phe 56.8 WT 52.1 WT 50.3 AAA/LysMC
L001 32 50.1 TTT/Phe 56.7 WT 47.7 TAC/TyrMC 56.6 WT
L002 8 50.0 TTT/Phe 56.4 WT 52.2 WT 56.8 WT
L003 ≥64 50.1 TTT/Phe 49.0 AAT/Asn 52.2 WT 50.3 AAA/Lys
L004 16 50.0 TTT/Phe 56.6 WT 44.9 TTC/Phe 56.8 WT
L005 32 49.5 TAT/Tyr 56.6 WT 52.2 WT 50.4 AAA/Lys
L006 16 50.1 TTT/Phe 56.8 WT 45.1 TTC/Phe 56.8 WT
L007 16 49.3 TAT/TyrMC 56.7 WT 52.3 WT 56.8 WT
L008 8 60.4 WT 56.7 WT 45.1 TTC/Phe 56.8 WT
L009 16 49.9 TTT/Phe 56.8 WT 45.0 TTC/Phe 56.8 WT
L010 32 49.9 TTT/Phe 56.7 WT 45.1 TTC/Phe 56.8 WT
DR22d 32 50.0 TTT/PheMC 56.5 WT 41.3 GCC/Phe 41.7 GGA/GluMC
SR27c 32 60.7 WT 48.5 TAT/TyrMC 44.5 TTC/Phe 56.6 WT
SR68c 32 36.8 AGA/ArgMC 56.5 WT 44.4 TTC/PheMC 56.8 WT
SR179c 8 36.6 AGA/Arg 56.6 WT 44.5 TTC/Phe 56.8 WT
SR248d 8 60.5 WT 48.7 AAT/AsnMC 41.2 GCC/AlaMC 56.6 WT
a

Strains N001 to N007 were isolated at Nagasaki University Hospital, and strains L001 to L010 were supplied by the Levofloxacin Surveillance Group.

b

Nucleotide sequence/amino acid sequence. Changed nucletides are shown in bold. WT, identical to the nucleotide distribution of the wild type, ATCC 49619. MC, mutant control.

c

Strains sourced from reference 22, supplied by Sapporo Medical University.

d

Strains sourced from reference 23, supplied by Sapporo Medical University.