TABLE 1.
Primers and probes
| Organism | Primers | Probes | Sequence (5′-3′) | Positiona |
|---|---|---|---|---|
| Rickettsia spp. | RCK/23-5-F | GATAGGTCRGRTGTGGAAGCAC | 1-22 (AY125012) | |
| RCK/23-5-R | TCGGGAYGGGATCGTGTGTTTC | 388-367 (AY125012) | ||
| Generic | GP-RICK | TAGCTCGATTGRTTTACTTTG | 51-71 (AY125012) | |
| SFG | GP-SFG | ACTCACAARGTTATCAGGT | 123-141 (AY125012) | |
| R. akari | P-AKA3 | GATCATGCAGCAATACATTAGC | 291105-291126 (AAFE01000001) | |
| R. belliii | P-BELL | GTGTTTATTCTATAATATGTCAG | 2721-2743 (U11015) | |
| R. slovaca | P-SLO | GTAGCCCCTGCCACGATA | 194-211 (AY125009) | |
| R. conorii | P-CON | GTTATATACTGTAGCCCTG | 186-204 (AY125012) | |
| R. aeschlimannii | P-AESCH | ATATTATACTGTATGTAGCCCC | 183-204 (AY125016) | |
| R. rickettsii-R. sibirica | P-RI/SI | GTTATACTGTAGTCCTGCAA | 2814-2833 (U11022) | |
| R. helvetica | P-HELV | CATGGCTTGATCCACGGTA | 360-342 (AY125017) | |
| R. felis | P-FEL | TAATGTTATACCGTGGTCCCGC | 186-207 (AM055829) | |
| R. australis | P-AUS | GACAAGTTTAGTTATGCAAT | 230-249 (AM055830) | |
| Typhus group | GP-TG | GTTATTCTATCGTTTTATGTYACG | 2804-2827 (U11018) | |
| R. prowazekii | P-PROW | TACGATTTGATAGTAAAGTTTTG | 2824-2846 (U11018) | |
| R. typhi (R. mooserii) | P-TYPHI | ATGTCACGATTTGACCGTAAGATC | 188-211 (AY125019) | |
| Cannabis sativa | P-CI2 | GTGGACACTTTAGTGGAGGAGG | 281-302 (AB183705) |
a
Accession numbers for the reference sequences are in parentheses.