TABLE 1.
Panel member | Concn
|
Mean concn
|
No.b | Precision
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Copies/ml | Log10 | Copies/ml | Log10 | SD (Log10)
|
CV (%)c
|
||||||
Interassay | Intraassay | Total | Interassay | Intraassay | Total | ||||||
1 | 30 | 1.5 | 31 | 1.5 | 10 | 0.18 | 0.25 | 0.31 | 45 | 61 | 81 |
2 | 50 | 1.7 | 47 | 1.7 | 34 | 0.12 | 0.25 | 0.28 | 28 | 74 | 84 |
3 | 100 | 2.0 | 82 | 1.9 | 47 | 0.08 | 0.20 | 0.21 | 18 | 48 | 52 |
4 | 1,000 | 3.0 | 916 | 3.0 | 48 | 0.10 | 0.11 | 0.14 | 23 | 25 | 34 |
5 | 10,000 | 4.0 | 9,665 | 4.0 | 47 | 0.11 | 0.07 | 0.13 | 26 | 17 | 31 |
6 | 100,000 | 5.0 | 125,050 | 5.1 | 48 | 0.14 | 0.08 | 0.16 | 33 | 17 | 38 |
7 | 1,000,000 | 6.0 | 1,621,759 | 6.2 | 48 | 0.16 | 0.14 | 0.22 | 41 | 34 | 56 |
8 | 5,000,000 | 6.7 | 6,227,011 | 6.8 | 43 | 0.02 | 0.17 | 0.17 | 5 | 42 | 43 |
Assessed with multiple replicates of an eight-member standard panel of HIV RNA with consecutive runs.
Number of replicates found.
CV, coefficient of variation.