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. 2006 Dec 15;20(24):3382–3394. doi: 10.1101/gad.1470906

Figure 3.

Figure 3.

Transcriptional derepression of Rhox6 and Rhox9 in DKO embryos. (A) Expression profile of some lineage markers in DKO embryos by RT–PCR. Total RNA was isolated from the E9.5 embryo proper (Emb), yolk sac (YS), and trophoblast (Troph). (Lane Norm.1) A Dnmt3a+/− embryo. (Lane Norm.2) A Dnmt3b+/− embryo. (Lanes DKO 3 and 4) Two independent Dnmt3a−/− Dnmt3b−/− embryos. (TE) Trophectoderm; (PrEnd) primitive endoderm; (ME) mesoderm. Gapdh was used as a control for equal RNA loading. PCR cycles were shown on the right. (B) Expression profiles obtained by RT–PCR in different DNA methyltransferase knockout embryos. Total RNA was isolated from the embryo proper of an E8.5 male. Xist and IAP-type I are known to be derepressed in DKO embryos (male) and Dnmt1−/− embryos, respectively (Walsh et al. 1998; Sado et al. 2004). (C) The expression profiles in female and male embryos by RT–PCR. Total RNA was isolated from E8.5 male (left) and female (right) embryos.