Figure 7.
Genetic interactions of AurA with Notch and aPKC. (A,A′) Notchts-1 suppresses the aurA overproliferation phenotype. Brains double mutant for Notchts-1; aurA8839 do not display overgrowth of NBs (A′) as indicated by the Dpn staining compared with aurA8839 (A) at 68 h ALH at 29°C. (B) Quantification of NB numbers per brain lobe for Notchts-1; aurA8839 and aurA8839 alone. (C–G) aPKC significantly suppresses NB overgrowth phenotype in aurA. Larval brains of wild-type (C), aPKC (D), aurA8839 (E), and aPKC; aurA8839 double mutant (F) were stained with Dpn and number of NBs per brain lobe were quantified at 68 h ALH (G). n = 20. (H) A model showing a pathway composed of AurA, aPKC, Numb, and Notch in the regulation of larval NB self-renewal. AurA is also involved in spindle orientation through regulating Mud asymmetric localization. However, in our model the proposed role for aPKC is formal and our genetic analysis does not imply a direct molecular role of AurA on aPKC.