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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: Biol Psychiatry. 2006 Apr 21;61(1):127–135. doi: 10.1016/j.biopsych.2006.03.002

Figure 1.

Figure 1

Schematic diagrams of the experimental paradigm and the brain regions analyzed for gene expression. (A)The experimental paradigm consisted of a 14-day training period in which all rats received one of two daily injections in two distinct environments (see Methods and Materials). Loco-motor activity was measured in context A over the training phase of the study. On the test day, 3 days following the final day of training, rats were reintroduced to context A with or without 5 minutes of ferret exposure immediately prior to reentry into context A. Locomotor activity was measured in context A for 45 minutes, after which time rats were sacrificed and trunk blood and brains were taken for plasma corticosterone assay and in situ hybridization analysis of immediate early gene expression in the pre-frontal and the primary motor cortices. (S.C. = saline cues, N.C. = nicotine cues). (B)The brain regions analyzed for activity-dependent gene expression via in situ hybridization included the prelimbic cortex (PreL), infralimbic cortex (InfrL), ventral orbital prefrontal cortex (VO), lateral orbital prefrontal cortex (LO), and the primary motor cortex (M1). Numbers represent distance from bregma in millimeters. S.C., saline cues; N.C., nicotine cues; PreL, prelimbic cortex; InfrL, infralimbic cortex; VO, ventral orbital prefrontal cortex; LO, lateral orbital prefrontal cortex; M1, primary motor cortex. (Adapted with permission from Paxinos and Watson 1998).