FIG. 1.
Generation of an NS-null allele and heterozygous NS-null (NS+/−) mice. (A) Schematic diagram of the NS genomic and protein structures. Boxes indicate its 15 exons. Coding exons for the basic (B), coiled-coil (CC), GTP-binding (GTP), and acidic (A) domains are shown in gray. (B) An NS-null allele was created by replacing the 640-bp proximal promoter and the first 7 1/2 exons with a neomycin selection cassette (neo). tk, thymidine kinase. (C) BamHI-digested genomic Southern blots of the heterozygous (+/−) and wild-type (+/+) F1 mice derived from the 5C and 9B chimeric founders showed a 5.2-kb or 6.3-kb fragment of the targeted allele when hybridized with the right-arm (RA) or left-arm (LA) probe. The 14.3-kb fragment represented the endogenous NS allele.