FIG. 2.
Expression of NS protein and population doublings were decreased in the NS+/− MEFs compared to the wild-type (WT) cells. (A) The expression levels of NS protein in the WT and NS+/− MEFs from P1 to P7 were determined in parallel by Western blot analyses using an anti-NS antibody (Ab2438). Anti-α-tubulin immunoblots were used as loading references (α-Tub). (B) The PDLs (y axis) in the NS+/− (black) and WT (gray) MEF cultures were plotted from P1 to P8 (x axis). PDLs were calculated using the formula ΔPDL = log(nf/n0)/log2, where n0 is the initial number of cells and nf is the final number of cells. Error bars represent standard errors of the means.