FIG. 6.

Overexpression of NS at a moderate level reduced the senescent cell percentage in the P6 and P7 MEF cultures. (A) Protein expression levels of NS in the WT and TG#1 MEFs from P1 to P6. Anti-α-tubulin immunoblots were used as loading controls (α-Tub). (B) PDLs of the TG#1 and WT MEF cultures over 8 passages was shown in black and gray, respectively. (C1) The percentages of SA-β-Gal⁺ cells were decreased in the TG#1 MEF cultures (black bars) compared to the WT cultures (gray bars) from P6 to P7. Error bars represent standard errors of the means in panels B and C1. Means (± standard errors of the means) and P values are shown in panel C2.