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. 2006 Oct 23;26(24):9149–9161. doi: 10.1128/MCB.00310-06

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FIG. 6. — PPH3 deletion strongly affects Rad53 dephosphorylation after UV irradiation. (A to D) Asynchronous cultures of wild-type (WT) (MCM185), mad2Δ (L48), ptc2Δ ptc3Δ (ptcΔ, L281), and pph3Δ (L289) cells were synchronized in G₁ with α-factor, washed thrice, and released into fresh medium in the presence of nocodazole. After 90 min, G₂/M-arrested cells were UV irradiated (40 J/m²) at time zero and further grown in fresh medium in the presence of nocodazole. Aliquots were taken at the indicated times after UV irradiation and analyzed by Western blotting using anti-Rad53 antibodies (A and C) and by FACS (B and D). (E and F) Asynchronous cultures of wild-type (MCM185), mad2Δ (L48), and pph3Δ (L289) cells were synchronized in G₁ with α-factor, washed thrice, and released into fresh medium (without nocodazole). After 90 min, the G₂/M-synchronized cells were UV irradiated (40 J/m²) at time zero. Samples were retrieved at the indicated times after UV irradiation and analyzed by Western blotting using anti-Rad53 antibodies (E) and by FACS (F).