FIG. 3.

FISC and βFtz-F1 are required for a proper 20E response in the fat body after blood ingestion. dsRNAs were injected into the thoraxes of female mosquitoes as described in Materials and Methods. The transcripts of the indicated genes in female mosquitoes were measured using real-time reverse transcription-PCR at the indicated time after blood feeding and were normalized to β-actin expression. WT, uninjected A. aegypti Rockefeller/UGAL strain; Mal RNAi, injected with double-stranded RNA complementary to bacterial malE; FISC RNAi, injected with FISC dsRNA; Ftz-F1 RNAi, injected with βFtz-F1 dsRNA. Arbitrary units are plotted against developmental time expressed as hours PBM. Representative data (means ± standard errors of the means) from at least three independent experiments are shown.