FIG. 3.

Functional interaction of dGcn5 and dAda2a. (A) Yeast two-hybrid assay indicates strong interaction between dAda2a and dGcn5. Constructs tested for interaction contained fusions (DNA-binding domain in pBTM and acidic activation domain in pGAD424) as follows: 1, dAda2a-none (negative control); 2, dRpb4-dRpb7 (positive control); 3, dAda2a-dAda3; 4, dAda2b-dAda3; 5, dAda2a-dGcn5; and 6, dADA2b-dGcn5. (B) The null dGcn5^E333st mutation has a negative effect on the development of dAda2a^hyp1. The phenotype change is shown as the fraction of animals reaching the adult (A), pharat adult (PhA), and early pupa (EP) stages in the wild-type (light gray bars) and dGcn5 heterozygous background (white bars). (C) The overexpression of dGcn5 from a transgene (dark gray bars) has a positive effect on the development of dAda2a^hyp2 (labels are as described in panel B). The inserts show the fractions of adults displaying the outstretched wing (os, white) and wild-type (wt, black) phenotypes. Resulting from the different chromosomal background (see Materials and Methods), the fractions of dAda2a^hyp1 and dAda2a^hyp2 adults in the two experiments (columns in panel B and C) are different. Error bars indicate deviations.