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. 2006 Oct 9;26(24):9430–9441. doi: 10.1128/MCB.01656-06

FIG. 3.

FIG. 3.

TCR mediated by different Rpb9 fragments. Gels show TCR in the GAL1 gene of log-phase rad16 rad26 (A), rad16 rad26 rpb9 (B), and rad16 rad26 rpb9 cells transformed with plasmids encoding full-length (residues 1 to 122) (C) and truncated (numbers indicate residues remaining) (D to K) Rpb9. Lanes U represent unirradiated samples. Lanes 0, 1, 2, and 4 indicate different times (hours) of repair incubation following UV irradiation. An arrow to the left of the gels marks the transcription start site. Solid circles on the left of the gels mark transcribed region (+1 to +380). Open triangles mark upstream region (−1 to −180) where residual Rpb9-mediated TCR takes place. Plots underneath each of the gels show the average (mean ± standard deviation) of the percent CPDs remaining at individual sites in the transcribed (+1 to +380) (solid circles) and upstream (−1 to −180) (open triangles) regions at different times of repair incubation.