FIG. 8.

Interaction of NEMOdel5 and NEMOdel7 with endogenous IKK subunits. Whole-cell extracts from NEMO-deficient Jurkat T cells transiently transfected with the indicated FLAG-NEMO constructs were subjected to an anti-FLAG immunoprecipitation and subsequently anti-IKKα, anti-IKKβ, and anti-NEMO immunoblot analysis (upper part). As a control for the equal expression of the different IKK subunits, a fraction of the whole-cell extracts was subjected to immunoblot analysis with the indicated antibodies (lower part). IB, immunoblot; IP, immunoprecipitate.