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. 2006 Oct 9;26(24):9338–9351. doi: 10.1128/MCB.01032-06

FIG. 8.

FIG. 8.

Irs-1 is inactivated in metastatic PyV-MT mammary tumors. Aliquots of tumor extracts from PyV-MT and PyV-MT::Irs2−/− mammary tumors containing equivalent amounts of total protein were immunoblotted with antibodies specific for total Irs-1 or phosphoserine Irs-1 (S302, S307, S636/S639, S789, and S1101) (upper panels). Aliquots of tumor extracts from WT and Irs2−/− transgenic mammary tumors containing equivalent amounts of total protein were immunoprecipitated with an Irs-1-specific antiserum and then immunoblotted with phosphotyrosine-specific antibodies. The immunoblots were subsequently stripped and reprobed with Irs-1-specific antibodies (lower panels). The numbers below indicate the relative levels of Irs-1 tyrosine phosphorylation in each tumor. WT, PyV-MT mice; Irs2−/−, PyV-MT::Irs2−/− mice.