Figure 5. .

Targeting of ectopically expressed HCCS wild-type and mutant proteins to mitochondria. Subcellular localization of different N-terminally HA-tagged HCCS proteins ectopically expressed in CHO-K1 cells (A, D, and G) and staining of endogenous mitochondria by MitoTracker (B, E, and H) are shown. HA-tagged HCCS wild-type protein (A [green]) is targeted to mitochondria (B [red]), as shown by colocalization with the MitoTracker (C [yellow]). Similarly, HA-tagged HCCS R217C mutant protein (D [green]) shows a mitochondrial (E [red]) distribution (F [yellow]). In contrast, the truncated HCCS Δ197–268 protein is diffusively dispersed in the cell (G), and the two fluorescence patterns (G and H) show no overlap (I). The scale bars represent 10 μm.