. 2006 Nov 28;400(Pt 3):541–550. doi: 10.1042/BJ20060766

Table 2. Values for K_m, k_cat and K_i for DQ, Q₂, DQH₂ and Q₂H₂ determined in the present study and compared with values reported previously.

Error values are 95% confidence intervals. CI, complex I.

DQ K_m (μM)	DQH₂K_i (μM)	Q₂K_m (μM)	Q₂H₂K_i (μM)	DQ k_cat (μmol·min⁻¹·mg⁻¹)	Q₂k_cat (μmol·min⁻¹·mg⁻¹)	k_cat(DQ)/k_cat(Q2)	Conditions	Reference
24.0±7.5	26.0±10	20.4±3.9	15.3±4.4	3.1±1.2	1.8±1.0	2.5±0.8	Isolated CI (pH 7.5), 100 μM NADH	This work
86	−	5	−	1.85	0.55	3.4	Isolated CI (pH 8.0), 5 μM NADH	[35]
6	−	2	−	0.45^*	0.21^*	2.1	SMPs (pH 7.4), 50 μM NADH	[40]
1.8	−	1.3	−	0.58±0.15^*	0.29^*	2.0	SMPs, 75 μM NADH	[31,34]

*Value reported per mg of total protein.

Table 2. Values for Km, kcat and Ki for DQ, Q2, DQH2 and Q2H2 determined in the present study and compared with values reported previously.