Figure 2. Concentration-dependent inhibition by Ht31 of AlphaScreen signal from AKAP95 (●) and AKAP149 (○) binding to RIIα.

In the assay, 1 nM biotinylated RIIα and 1 nM GST–AKAP were used with Ht31 peptide concentrations ranging from 2000 to 0.12 nM. For experimental conditions, see the Materials and methods section. The IC₅₀ values were calculated for each AKAP by non-linear regression analysis using SigmaPlot. Results are means±S.E.M. for three independent experiments performed in duplicate.