Figure 3. Distribution analysis of known and novel proteins in DRM.

(A) Equal aliquots of each sucrose-gradient fraction were separated by SDS/PAGE and immunoblotted for several known and novel identified proteins in DRM, illustrating the varying degree of relative enrichment (indicated as a percentage of total signal). (B) Confluent HUVEC were fixed and analysed by confocal microscopy to reveal the cellular distribution of each protein using specific antibodies. Indicated are the PM (arrows) and the Golgi apparatus (arrowheads). Scale bars represent 10 μm.